A set of primers for amplification of mitochondrial DNA in Picea abies and other conifer species

Authors

  • S. Jeandroz,

    1. UMR INRA/UHP 1136 Tree–Microbe Interactions. Université Henry Poincaré Nancy I. 54506 Vandoeuvre lès Nancy, France,
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  • D. Bastien,

    1. UMR INRA/UHP 1136 Tree–Microbe Interactions. Université Henry Poincaré Nancy I. 54506 Vandoeuvre lès Nancy, France,
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  • A. Chandelier,

    1. Département de Biochimie et Biologie Appliquées, Unité de Biologie Végétale, Faculté Universitaire des Sciences Agronomiques de Gembloux, B-5030 Gembloux, Belgium
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  • P. Du Jardin,

    1. Département de Biochimie et Biologie Appliquées, Unité de Biologie Végétale, Faculté Universitaire des Sciences Agronomiques de Gembloux, B-5030 Gembloux, Belgium
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  • J. M. Favre

    Corresponding author
    1. UMR INRA/UHP 1136 Tree–Microbe Interactions. Université Henry Poincaré Nancy I. 54506 Vandoeuvre lès Nancy, France,
      Jean-Michel Favre. Fax: (33) 3 83 90 32 77; E-mail: favre@scbiol.u-nancy.fr
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Jean-Michel Favre. Fax: (33) 3 83 90 32 77; E-mail: favre@scbiol.u-nancy.fr

Abstract

Thirteen primer pairs generating intraspecific length and/or presence-absence polymorphism in Picea abies have been obtained from a P. abies mtDNA library, using different methodologies (agarose gel electrophoresis, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), single-strand conformation (SSCP). Poly-morphism tests were extended successfully to other Picea species (P. omorika, P. engelmanii and P. glauca) and species belonging to other conifer genera (Abies alba, Larix laricina and Pinus pinaster). This set of PCR-based mitochondrial markers can provide promising tools for studying phylogeography or phylogeny in P. abies and other conifer species in which the mt genome is generally the only one to be transmitted via the female gamete.

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