Isolation and characterization of polymorphic microsatellites in the Trinidadian guppy (Poecilia reticulata)


S. A. Becher. *Present address: Oxford University Bioinformatics Centre, Sir William Dunn School of Pathology, South Parks Road, Oxford OX1 3RE, UK. Fax: + 44–1865–275501; E-mail:


We isolated microsatellite loci for the Trinidadian guppy Poecilia reticulata from the Oropuche and Caroni drainages in Trinidad, using a modified enrichment protocol that provided 93% enriched libraries. The eight loci presented here are polymorphic, and have between seven and 18 alleles in 33–48 individuals initially screened. Observed heterozygosities in this sample range from 0.28 to 0.88. These loci significantly increase the number of polymorphic microsatellite markers available for paternity and population analysis in this species.