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Isolation and characterization of microsatellite DNA markers in the malaria vector Anopheles funestus

Authors

  • A. Cohuet,

    Corresponding author
    1. Laboratoire de Lutte contre les Insectes Nuisibles (LIN), Institut de Recherche pour le Développement (IRD), BP 64501, 34394 Montpellier Cedex 05, France,
      Anna Cohuet. Fax: + 33 4 67 54 20 44; E-mail: anna.cohuet@mpl.ird.fr
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  • F. Simard,

    1. Laboratoire IRD d’Entomologie Médicale, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC), BP 288, Yaoundé, Cameroun,
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  • A. Berthomieu,

    1. Institut des Sciences de l’Evolution, Laboratoire de Génétique et Environnement, CC065, UMR CNRS 5554, Université de Montpellier II, France
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  • M. Raymond,

    1. Institut des Sciences de l’Evolution, Laboratoire de Génétique et Environnement, CC065, UMR CNRS 5554, Université de Montpellier II, France
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  • D. Fontenille,

    1. Laboratoire de Lutte contre les Insectes Nuisibles (LIN), Institut de Recherche pour le Développement (IRD), BP 64501, 34394 Montpellier Cedex 05, France,
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  • M. Weill

    1. Institut des Sciences de l’Evolution, Laboratoire de Génétique et Environnement, CC065, UMR CNRS 5554, Université de Montpellier II, France
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Anna Cohuet. Fax: + 33 4 67 54 20 44; E-mail: anna.cohuet@mpl.ird.fr

Abstract

Screening of the Anopheles funestus genomic DNA library detected 18 new sequences with dinucleotide tandem repeats. Primers were designed to amplify the loci and 14 out of 18 gave a repeatable and scorable amplification. Deviations from Hardy–Weinberg expectations were tested for each locus in a sample of 30 wild Anopheles funestus females. No heterozygote deficiency was detected for 11 loci of 14, thus revealing the absence of null alleles. The number of alleles per locus ranged from 5 to 15, and observed heterozygosity from 0.13 to 0.85.

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