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Dinucleotide repeat microsatellite markers for buck’s-horn plantain (Plantago coronopus)

Authors

  • G. N. Koorevaar,

    Corresponding author
    1. Netherlands Institute of Ecology, Department of Plant Population Biology, Boterhoeksestraat 48, PO Box 40, 6666 ZG Heteren, The Netherlands,
      G. Koorevaar. Fax: + 31 26 4723227; E-mail: Koorevaar@cto.nioo.knaw.nl
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  • S. Ivanovic,

    1. Netherlands Institute of Ecology, Department of Plant Population Biology, Boterhoeksestraat 48, PO Box 40, 6666 ZG Heteren, The Netherlands,
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  • J. M. M. Van Damme,

    1. Netherlands Institute of Ecology, Department of Plant Population Biology, Boterhoeksestraat 48, PO Box 40, 6666 ZG Heteren, The Netherlands,
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  • H. P. Koelewijn,

    1. Netherlands Institute of Ecology, Department of Plant Population Biology, Boterhoeksestraat 48, PO Box 40, 6666 ZG Heteren, The Netherlands,
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  • W. P. C. Van’T Westende,

    1. Plant Research International, Department of Biodiversity and Identity, PO Box 16, 6700 AA Wageningen, the Netherlands
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  • M. J. M. Smulders,

    1. Plant Research International, Department of Biodiversity and Identity, PO Box 16, 6700 AA Wageningen, the Netherlands
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  • B. Vosman

    1. Plant Research International, Department of Biodiversity and Identity, PO Box 16, 6700 AA Wageningen, the Netherlands
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G. Koorevaar. Fax: + 31 26 4723227; E-mail: Koorevaar@cto.nioo.knaw.nl

Abstract

Eleven polymorphic microsatellite loci were obtained from a GA enriched genomic library, constructed from DNA of buck’s-horn plantain (Plantago coronopus). The microsatellite loci were tested on 24 genotypes. These plants were collected from meadows along the coast, located on 11 sites ranging from the southwest to the northeast of the Netherlands. In this set of plants the isolated microsatellites were highly polymorphic with 3–24 alleles per locus and a maximum observed heterozygosity of 0.91. Some of the microsatellite loci also showed amplification in two other plantain species (P. lanceolata and P. maritima).

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