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Characterization of microsatellite loci in Anopheles flavirostris, the principal malaria vector in the Philippines

Authors

  • E. P. Torres,

    Corresponding author
    1. Department of Medical Entomology, Research Institute for Tropical Medicine (RITM), Department of Health, Filinvest Corporate City Compound, Alabang Muntinlupa City, Metro Manila 1770, Philippines,
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  • K. Fischer,

    1. Malaria Biology Laboratory, The Queensland Institute of Medical Research (QIMR), The Bancroft Centre, 300 Herston Road Brisbane, Queensland 4029, Australia,
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  • D. H. Foley,

    1. Tropical Health Program and the Department of Zoology and Entomology, University of Queensland, Brisbane Queensland 4072, Australia
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  • D. Kemp

    1. Malaria Biology Laboratory, The Queensland Institute of Medical Research (QIMR), The Bancroft Centre, 300 Herston Road Brisbane, Queensland 4029, Australia,
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E.P. Torres. Fax: (632) 842 2245, E-mail: etorres@ritm.gov.ph

Abstract

Microsatellites were isolated and characterized from Anopheles flavirostris, the principal malaria vector in the Philippines. Fifty of the 150 positive clones sequenced contained mostly dinucleotide microsatellites and only 16 had trinucleotide repeats. We designed primers from the unique sequences flanking 18 microsatellite loci. Of these, 11 loci produced successful amplification and revealed high levels of polymorphism; 86 alleles were detected with allele number ranging from 2 to 16 at each locus. The high allelic variability will make these microsatellite loci very useful for taxonomic and population genetic studies.

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