Development and characterization of microsatellite markers for Liporrhopalum tentacularis Grandi, the pollinator fig wasp of Ficus montana Blume

Authors


M. Zavodna. ‡Present address (until April 2003): Centre for Plant Sciences, School of Biology, Irene Manton Building, University of Leeds, Leeds, LS2 9JT, U.K. Fax: +44 1133433144; E-mail: z_monika@hotmail.com

Abstract

Microsatellite markers for the pollinator fig wasp Liporrhopalum tentacularis were developed using genomic libraries enriched for di-, tri- and tetranucleotide repeats. A subset of 31 positive clones was sequenced and primers were designed. Eleven primer pairs produced polymorphic amplification products in L. tentacularis. Eight markers gave unambiguously scorable patterns and were further characterized on 29 individuals collected from different fruits of the dioecious host fig Ficus montana in Indonesia. Three to 19 alleles per locus were detected in this set of samples. The observed heterozygosity ranged between 0.10 and 0.55.

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