Isolation and characterization of microsatellite DNA primers in burrowing owl (Athene cunicularia)

Authors


Nicole M. Korfanta. Fax: (307) 766 5099; E-mail: korfanta@uwyo.edu

Abstract

We developed polymerase chain reaction (PCR) primers to amplify 22 microsatellite DNA loci in burrowing owls (Strigiformes: Athene cunicularia). The seven most easily amplified and scored polymorphic loci are presented. These show 3−19 alleles per locus and expected heterozygosities of 0.067–0.712. Inheritance at all loci conformed to Mendelian expectations among six owl families, and six loci conform to Hardy–Weinberg expectations.

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