Microsatellite DNA loci from the Diamondback terrapin (Malaclemys terrapin)

  1. J. Susanne Hauswaldt1,2,*,
  2. Travis C. Glenn1,2

Article first published online: 2 APR 2003

DOI: 10.1046/j.1471-8286.2003.00388.x

Issue

Molecular Ecology Notes

Molecular Ecology Notes

Volume 3, Issue 2, pages 174–176, June 2003

Additional Information

How to Cite

Hauswaldt, J. S. and Glenn, T. C. (2003), Microsatellite DNA loci from the Diamondback terrapin (Malaclemys terrapin). Molecular Ecology Notes, 3: 174–176. doi: 10.1046/j.1471-8286.2003.00388.x

Author Information

  1. 1

    Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA,

  2. 2

    Savannah River Ecology Laboratory, P.O. Drawer E, Aiken, SC 29802, USA

*Susanne Hauswaldt. Fax: 803-777-4002; E-mail: sushaus@biol.sc.edu

Publication History

  1. Issue published online: 2 APR 2003
  2. Article first published online: 2 APR 2003
  3. Received 3 October 2002; revision received 18 December 2002; accepted 18 December 2002

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Keywords:

  • dinucleotide repeats;
  • Malaclemys terrapin;
  • microsatellites;
  • primer;
  • SSR;
  • tetranucleotide repeats

Abstract

We describe polymerase chain recation (PCR) primers and conditions to amplify one dinucleotide and five tetranucleotide microsatellite DNA loci isolated from the Diamondback terrapin (Malaclemys terrapin). The PCR primers were tested on 21 terrapins from Cape Romain, SC, USA. The microsatellite primers developed yielded a high number of alleles (8–14) and high observed heterozygosities (0.57–1.0).

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