New tetranucleotide microsatellites for fine-scale discrimination among endangered chinook salmon (Oncorhynchus tshawytscha)

Authors

  • Carolyn Greig,

    Corresponding author
    1. Wellcome Trust Center for Cell Matrix Research, University of Manchester, Oxford Road, Manchester M13 2PT, UK,
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  • David P. Jacobson,

    1. Coastal Oregon Maine Experiment Station, Oregon State University Hatfield Marine Science Center, 2030 SE Marine Science Drive, Newport, OR 97365, USA
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  • Michael A. Banks

    1. Coastal Oregon Maine Experiment Station, Oregon State University Hatfield Marine Science Center, 2030 SE Marine Science Drive, Newport, OR 97365, USA
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Carolyn Greig. Fax: + 44 161 275 5082; E-mail: carolyn.greig@man.ac.uk

Abstract

The unambiguous identification of Central Valley spring-run chinook salmon has become imperative since their proposed listing in 1998. The accuracy of methods used to assign individuals to their stock of origin is critical for understanding juvenile migration patterns and determining the success of protection measures. Existing microsatellites discriminate between the endangered winter-run and other chinook but are insufficient to characterize phylogenetically less distinct runs. Here, we isolated and developed highly variable tetranucleotide microsatellites for the specific goal of increasing discriminatory power among closely related populations, providing a new power towards the reliable differentiation of nonwinter runs

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