Isolation and characterization of polymorphic microsatellite loci from the Dungeness crab Cancer magister

Authors

  • Robert J. Toonen,

    Corresponding author
    1. Center for Population Biology, Section of Evolution & Ecology, University of California, Davis, CA 95616 USA,
    2. Hawaii Institute of Marine Biology, University of Hawaii at Manoa, Kaneohe, HI 96744 USA
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  • Melissa Locke,

    1. Center for Population Biology, Section of Evolution & Ecology, University of California, Davis, CA 95616 USA,
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  • Rick Grosberg

    1. Center for Population Biology, Section of Evolution & Ecology, University of California, Davis, CA 95616 USA,
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Rob Toonen, Hawaii Institute of Marine Biology, School of Ocean & Earth Science & Technology, University of Hawaii, Kaneohe, HI 96744 USA. Fax: (808) 236 7443; E-mail: toonen@hawaii.edu

Abstract

The Dungeness crab, Cancer magister, is the focus of one of the most intensely harvested fisheries in North America. Given its economic importance, there is considerable interest in assessing the degree and spatial pattern of genetic structure in C. magister. To that end, we developed a series of 17 hypervariable microsatellite loci. Six of these 17 loci could be amplified in a single multiplex PCR reaction. Using dye-labelled primers all six loci can be coamplified and scored simultaneously on an automated sequencer. The ability to multiplex multiple loci greatly increases the ease and speed of genotyping for this species.

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