Isolation and characterization of polymorphic microsatellite DNA markers in topmouth gudgeon, Pseudorasbora (Teleostei: Cyprinidae)

Authors

  • M. Konishi,

    Corresponding author
    1. Department of Environmental System Science, Graduate School of Science and Technology, Shinshu University, 3-1-1, Asahi, Matsumoto, Nagano 390–8621, Japan
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  • K. Takata

    1. Department of Environmental System Science, Graduate School of Science and Technology, Shinshu University, 3-1-1, Asahi, Matsumoto, Nagano 390–8621, Japan
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Mayu Konishi. Fax: + 81-263-37-2560; E-mail: t00h104@amail.shinshu-u.ac.jp

Abstract

Due to accidental introductions, the distribution of the east Asian topmouth gudgeon, Pseudorasbora parva, has rapidly expanded over recent years, thereby threatening the endangered Japanese species, P. pumila pumila. Sixteen microsatellite loci were isolated and characterized for P. parva, 14 being highly polymorphic (mean HE = 0.58, mean number of alleles = 4.4). Successful characterization of 10 of these loci was also achieved for P. pumila pumila. These markers should be useful in future investigation of colonization of P. parva and the development of conservation strategies for P. pumila pumila.

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