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Identification and characterization of microsatellite DNA markers developed in ide Leuciscus idus and Siberian roach Rutilus rutilus

Authors

  • Anna Barinova,

    1. Laboratory of Applied Population Genetic Informatics, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan,
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  • Elena Yadrenkina,

    1. Institute of Animal Systematics and Ecology, Siberian Branch, Russian Academy of Sciences, Novosibirsk 630091, Russia
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  • Masamichi Nakajima,

    1. Laboratory of Applied Population Genetic Informatics, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan,
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  • Nobuhiko Taniguchi

    Corresponding author
    1. Laboratory of Applied Population Genetic Informatics, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan,
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Prof. Nobuhiko Taniguchi. Fax: 81-22-717-8743; E-mail: nobuhiko@bios.tohoku.ac.jp

Abstract

The first five microsatellite markers for the ide, Leuciscus idus, and four microsatellite markers for the Siberian roach, Rutilus rutilus, were designed. Cross-amplification of ide markers was examined in Siberian roach and vice versa. The number of alleles per locus ranged from three to 13 in ide and from two to eight in roach. The expected heterozygosity ranged from 0.313 to 0.909 in ide and from 0.119 to 0.775 in roach. These markers could be used to evaluate the genetic population structure of these species and other fish from the Cyprinidae family.

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