Present address: Institute of Water and Environment, Cranfield University, Silsoe, Bedford MK45 4DT, UK.
Polymorphic microsatellite loci in the black-and-gold chromis, Neoglyphidodon nigroris (Teleostei: Pomacentridae)
Article first published online: 6 JAN 2004
Molecular Ecology Notes
Volume 4, Issue 1, pages 93–95, March 2004
How to Cite
Watts, P. C., Veltsos, P., Soffa, B. J., Gill, A. B. and Kemp, S. J. (2004), Polymorphic microsatellite loci in the black-and-gold chromis, Neoglyphidodon nigroris (Teleostei: Pomacentridae). Molecular Ecology Notes, 4: 93–95. doi: 10.1046/j.1471-8286.2003.00579.x
- Issue published online: 6 JAN 2004
- Article first published online: 6 JAN 2004
- Received 6 October 2003; revision accepted 14 November 2003
- black-and-gold chromis;
- Neoglyphidodon nigroris
We describe 15 polymorphic dinucleotide microsatellite loci in the black-and-gold chromis, Neoglyphidodon nigroris (Cuvier: Teleostei: Pomacentridae). Microsatellites were isolated from a partial genomic library that was enriched for CA repeat motifs. The 15 loci yielded between two and 23 alleles per locus in a sample of 16 fish from two forereef sites off the island of Hoga, Wakatobi National Park, Indonesia. Observed and expected heterozygosities varied from 0.13 to 1.00 and 0.24 to 0.98, respectively. These markers should allow us to discriminate between closely related individuals and also to assess the connectedness of populations of N. nigroris that inhabit different reefs.