A simple artificial urine for the growth of urinary pathogens

Authors


Dr C. William Keevil Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 0JG, UK

Abstract

A simple artificial urine medium (AUM) has been developed which provides conditions similar to that found in human urine. AUM solidified with agar enabled the recovery of a wide range of urease-positive and -negative urinary pathogens. Liquid AUM supported growth at concentrations of up to 108 cfu ml−1, as found in normal urine. Reproducible, steady-state growth also occurred over many generations in continuous culture. AUM was capable of forming crystals and encrustations resembling those found in natural urinary tract infections. The medium is a suitable replacement for normal urine for use in a wide range of experiments modelling the growth and attachment of urinary pathogens in the clinical environment.

Footnotes

  1. Present address : Chemical and Biological Defence Establishment, Porton Down, Salisbury SP4 0JG, UK.

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