Development of an inducible gene expression system for Lactobacillus sakei
Article first published online: 11 JUL 2003
Letters in Applied Microbiology
Volume 37, Issue 2, pages 115–120, August 2003
How to Cite
Axelsson, L., Lindstad, G. and Naterstad, K. (2003), Development of an inducible gene expression system for Lactobacillus sakei. Letters in Applied Microbiology, 37: 115–120. doi: 10.1046/j.1472-765X.2003.01360.x
- Issue published online: 11 JUL 2003
- Article first published online: 11 JUL 2003
- 2003/0096: received 5 February 2003, revised 7 March 2003 and accepted 7 April 2003
- controlled expression;
Aim: To develop an inducible gene expression system for Lactobacillus sakei, based on the regulatory system of sakacin A production.
Methods and Results: A Lactobacillus/Escherichia coli shuttle vector; pKRV3, was constructed including the signal transducing system genes of the bacteriocin sakacin A. The gusA gene fused to PsapA promoter, cloned in this vector allowed for inducible β-glucuronidase expression in L. sakei and L. plantarum following the addition of the sakacin A inducing peptide. PsapA appeared to be a strong and tightly controlled promoter when compared with known promoters.
Conclusion: The pKRV3 system can be used as an inducible gene expression system in lactobacilli.
Significance and Impact of the Study: A novel, inducible gene expression system has been developed for lactic acid bacteria relevant in food fermentations.