A study of the yeast cell wall composition and structure in response to growth conditions and mode of cultivation
Article first published online: 6 AUG 2003
Letters in Applied Microbiology
Volume 37, Issue 3, pages 268–274, September 2003
How to Cite
Aguilar-Uscanga, B. and François, J.M. (2003), A study of the yeast cell wall composition and structure in response to growth conditions and mode of cultivation. Letters in Applied Microbiology, 37: 268–274. doi: 10.1046/j.1472-765X.2003.01394.x
- Issue published online: 6 AUG 2003
- Article first published online: 6 AUG 2003
- 2003/0265: received 1 March 2003, revised 18 June 2003 and accepted 19 June 2003
Aim: The polysaccharide composition of the Saccharomyces cerevisiae cell wall was measured under various growth conditions and was compared with the cell wall structure.
Methods and Results: Chemical and enzymatic methods were used to determine levels of β-1,3-glucan and 1,6-glucan, mannan and chitin of the yeast cell wall, whereas the structure/resistance of the wall was qualitatively assessed by the sensibility to the lytic action by zymolyase. It was found that the dry mass and polysaccharides content of the cell wall could vary by more than 50% with the nature of the carbon source, nitrogen limitation, pH, temperature and aeration, and with the mode of cell cultivation (shake flasks vs controlled fermentors). While no obvious correlation could be found between β-glucan or mannan levels and the susceptibility of whole yeast cells to zymolyase, increase of β-1,6-glucan levels, albeit modest with respect to the growth conditions investigated, and to a lesser extent that of chitin, was associated with decreased sensitivity of yeast cells to the lytic action by zymolyase.
Significance and Impact of the Study: Our results indicate that the cell wall structure is merely determined by cross-linking between cell wall polymers, pointed out the role of β-1,6-glucan in this process. Hence, this study reinforces the idea that enzymes involved in these cross-linking reactions are potential targets for antifungal drugs.