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Expression of Bacillus subtilis phytase in Lactobacillus plantarum 755

  1. J. Kerovuo1,
  2. S. Tynkkynen2

Article first published online: 25 DEC 2001

DOI: 10.1046/j.1472-765x.2000.00660.x

Issue

Letters in Applied Microbiology

Letters in Applied Microbiology

Volume 30, Issue 4, pages 325–329, April 2000

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How to Cite

Kerovuo, J. and Tynkkynen, S. (2000), Expression of Bacillus subtilis phytase in Lactobacillus plantarum 755. Letters in Applied Microbiology, 30: 325–329. doi: 10.1046/j.1472-765x.2000.00660.x

Author Information

  1. 1

    Danisco Finland, Technology Center, Kantvik and

  2. 2

    Valio Ltd, Research and Development Center, Helsinki, Finland

* J. Kerovuo, Danisco Finland, Kantvik Research Center, FIN-02460 Kantvik, Finland (e-mail: janne.kerovuo@cultor.com).

Publication History

  1. Issue published online: 25 DEC 2001
  2. Article first published online: 25 DEC 2001
  3. 0008/99: received 16 December 1999 and accepted 10 January 2000

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Phytase enzymes can increase the nutritional value of food and feed by liberating inorganic phosphate from phytate, the major storage form of phosphorus in plants. The phytase (phyC) from Bacillus subtilis VTT E-68013 was expressed in Lactobacillus plantarum strain 755 using Lact. amylovorusα-amylase secretion signals. In an overnight cultivation in MRS medium containing cellobiose for induction of the α-amylase promoter, catalytically active phytase was secreted as a predominant extracellular protein. However, Western blot analysis revealed unprocessed and processed phytase in the cell fraction. Pulse chase experiments showed that the recombinant phytase was secreted at a slower rate in comparison to the native proteins of Lact. plantarum 755.

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