Bacillus cereus produces most emetic toxin at lower temperatures


Dr A.D. Sutherland, School of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 OBA, UK (e-mail:


Seven emetic toxin-producing strains of Bacillus cereus were examined for toxin production in Skim Milk Medium at incubation temperatures ranging from 10 to 50 °C. Minimum and maximum growth temperatures were found to be 12 and 46 °C, respectively. At 12 and 15 °C, levels of toxin production were significantly higher (P < 0·01) than that observed at 30 °C, while no toxin was produced above 37 °C. Increased levels of sporulation were observed at increased temperatures, and no correlation was found between levels of sporulation and toxin production (R2 = 0·086).