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Seven emetic toxin-producing strains of Bacillus cereus were examined for toxin production in Skim Milk Medium at incubation temperatures ranging from 10 to 50 °C. Minimum and maximum growth temperatures were found to be 12 and 46 °C, respectively. At 12 and 15 °C, levels of toxin production were significantly higher (P < 0·01) than that observed at 30 °C, while no toxin was produced above 37 °C. Increased levels of sporulation were observed at increased temperatures, and no correlation was found between levels of sporulation and toxin production (R2 = 0·086).