Lactobacillus plantarum phytase activity is due to non-specific acid phosphatase


Dr M. Zamudio Maya, Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Av. Juárez no. 421 Cd. Industrial C.P. 97288 Apdo. Postal 1226-A, Mérida, Yucatán, México (e-mail:


M. ZAMUDIO, A. GONZÁLEZ AND J.A. MEDINA. 2001. Microbial phytases suitable for food fermentations could be obtained from lactic acid bacteria isolated from natural vegetable fermentations. Phytase activity was evaluated for six lactic acid bacteria cultures. Although the highest activity was found for Lactobacillus plantarum, the phytase activity was very low. Further characterization of the enzyme with phytate-degrading activity showed a molecular weight of 52 kDa and an optimum activity at pH 5·5 and 65°C. Enzyme activity was due to a non-specific acid phosphatase which had a higher hydrolysis rate with monophosphorylated compounds such as acetyl phosphate that could explain the low phytase activity.