PCR-ELISA detection of Escherichia coli in milk
Article first published online: 1 MAR 2002
Letters in Applied Microbiology
Volume 34, Issue 3, pages 222–226, March 2002
How to Cite
Daly, P. , Collier, T. and Doyle, S. (2002), PCR-ELISA detection of Escherichia coli in milk. Letters in Applied Microbiology, 34: 222–226. doi: 10.1046/j.1472-765x.2002.01074.x
- Issue published online: 1 MAR 2002
- Article first published online: 1 MAR 2002
Aims: The purpose of this study was to develop a reliable molecular procedure for the detection of Escherichia coli in milk.
Methods and Results: Robust and expeditious DNA extraction and PCR techniques were evaluated using Enzyme-Linked Immunosorbent Assay (ELISA) detection of biotin-labelled amplicons to facilitate optimal detection of E. coli DNA.
Conclusions: It was found that 5 E. coli colony-forming units (cfu) could be detected per PCR reaction using the PCR-ELISA system, equating to a sensitivity of detection of 100 E. coli cfu ml−1 pasteurized milk.
Significance and Impact of the Study: This approach should facilitate evaluation of milk contamination and enable rapid detection of E. coli mastitis, leading to correct deployment of relevant antibiotic therapy and improved animal welfare.