It is well documented that myopia is associated with an increase in axial length of the posterior vitreous chamber. Whether equatorial or transverse dimensions are likewise affected in myopia is relevant to further understanding of the development of ametropia. We have utilised a computing method to determine retinal contour from real eye measurements of keratometry, A-scan ultrasonography and peripheral refraction as a means of assessing the transverse dimensions of the vitreous chamber. This technique has been applied to a 21-year-old female Caucasian anisomyope with a refractive error of R −1.50/−0.50 × 130 and L −4.00/−0.50 × 160. Anisomyopia offers a special opportunity for inter-eye comparison of different degrees of myopia. The repeatability of the technique was assessed by taking 10 separate sets of the aforementioned measurements and thus generating 10 retinal contours for each eye. We conclude that this method is repeatable and is capable of demonstrating differences between anisomyopic eyes although validation against in vivo measurements is required.