Evidence of Absence of Porcine Endogenous Retrovirus (PERV) Infection in Patients Treated with a Bioartificial Liver Support System
Article first published online: 24 DEC 2001
Volume 23, Issue 9, pages 829–833, September 1999
How to Cite
Pitkin, Z. and Mullon, C. (1999), Evidence of Absence of Porcine Endogenous Retrovirus (PERV) Infection in Patients Treated with a Bioartificial Liver Support System. Artificial Organs, 23: 829–833. doi: 10.1046/j.1525-1594.1999.06444.x
- Issue published online: 24 DEC 2001
- Article first published online: 24 DEC 2001
- Received March 1999; revised May 1999.
- Bioartificial liver;
- Porcine hepatocytes;
Abstract: Porcine endogenous retrovirus (PERV) genomes are present in all pig cells. In this retrospective study, we assessed PERV infectivity in 28 patients treated with an extracorporeal bioartificial liver (HepatAssist system) that includes a membrane device containing porcine hepatocytes. All patients tested negative for PERV using polymerase chain reaction analysis of peripheral blood mononuclear cells (PBMC) collected up to 5 years after treatment. In vitro results showed that the membrane decreased the risk of PERV transmission by a factor of 105, and porcine hepatocytes did not produce infectious PERV in co-cultures with human cell line 293. Our results do not support the presence of PERV infection in patients treated with this porcine hepatocyte-based bioartificial liver.