It is the goal of this section to publish material that provides information regarding specific issues, aspects of artificial organ application, approach, philosophy, suggestions, and/or thoughts for the future.
Functional Analysis of the Tissue-Engineered Stomach Wall
Article first published online: 25 SEP 2002
Volume 26, Issue 10, pages 868–872, October 2002
How to Cite
Hori, Y., Nakamura, T., Kimura, D., Kaino, K., Kurokawa, Y., Satomi, S. and Shimizu, Y. (2002), Functional Analysis of the Tissue-Engineered Stomach Wall. Artificial Organs, 26: 868–872. doi: 10.1046/j.1525-1594.2002.07006.x
- Issue published online: 25 SEP 2002
- Article first published online: 25 SEP 2002
- Received November 2001; revised May 2002.
- Gastrointestinal tract;
- Regenerative medicine;
- Tissue engineering
Abstract: We have established a method for in situ tissue engineering of the stomach in a canine model using an acellular collagen scaffold graft. The current study was conducted to evaluate the functional aspects of the tissue-engineered stomach wall. The anterior wall of the stomach in beagle dogs was replaced with a collagen sponge scaffold measuring 4 × 4 cm. At 16 weeks after implantation, the animals were sacrificed and the stomach specimens were evaluated immunohistochemically and physiologically. Regeneration of the proton pump and thin muscle layer, which are essential for mechanical and chemical digestion by the stomach, was observed in the tissue-engineered gastric tissue. However, acetylcholine-induced contraction was not observed in the tissue-engineered stomach wall. Although there is still room for improvement, the tissue-engineered stomach wall had a highly organized structure, and it is anticipated that this approach could eventually become an alternative for stomach reconstruction after gastrectomy.