Plasticity of 5-HT2A Serotonin Receptor in Patients With Analgesic-Induced Transformed Migraine

Authors

  • Anan Srikiatkhachorn MD,

    Corresponding author
    1. From the Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand (Dr. Srikiatkhachorn) and the
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  • Supaporn Puangniyom MS,

    1. Neuro- and Behavioural Biology Center, Institute of Science and Technology for Research and Development, Mahidol University, Salaya, Nakornpathom, Thailand (Miss Puangniyom and Dr. Govitrapong).
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  • Piyarat Govitrapong PhD

    1. Neuro- and Behavioural Biology Center, Institute of Science and Technology for Research and Development, Mahidol University, Salaya, Nakornpathom, Thailand (Miss Puangniyom and Dr. Govitrapong).
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Address all correspondence to Dr. Anan Srikiatkhachorn, Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.

Abstract

Chronic drug exposure can induce a significant change in neurotransmitter receptor systems and is possibly involved in the pathogenesis of drug-induced neurological disorders. Abuse of analgesics is known to induce deterioration in headache status in patients with primary headaches, especially migraine. To assess the possibility of 5-HT2A serotonin receptor plasticity in this condition, we investigated receptor binding on the platelet membrane in patients with analgesic-induced transformed migraine, patients with migraine, and nonheadache controls. Various concentrations of [3H]-spiperone (0.4 to 12 nmol) was used as a radioligand, and ketanserin was used to determine nonspecific binding. A lower maximal number of receptors (Bmax) was observed in patients with migraine as compared to patients with transformed migraine, and controls (467 ± 58, 708 ± 36, and 786 ± 64 fmol/mg protein, respectively, P<0.01); whereas the value of the dissociation equilibrium constant (Kd) remained unchanged (1.72 ± 0.16, 1.41 ± 0.13, and 1.25 ± 0.21 nmol for patients with migraine, patients with transformed migraine, and nonheadache controls, respectively). A significant decrease in Bmax value was observed in patients with transformed migraine after 4 weeks of analgesic withdrawal (770 ± 25 and 345 ± 31 fmol/mg protein, P<0.001), whilst no significant change in Kd value was observed (1.95 ± 0.12 and 2.47 ± 0.30 nmol, respectively). These findings indicate that 5-HT2A serotonin receptor system is altered in patients with transformed migraine with analgesic overuse. Such receptor plasticity may be an important step in the pathogenic mechanism of transformed migraine.

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