Acetoacetate, Acetone, and Dibenzylamine (a Contaminant in l-(+)-β-Hydroxybutyrate) Exhibit Direct Anticonvulsant Actions in Vivo
Article first published online: 22 APR 2002
Volume 43, Issue 4, pages 358–361, April 2002
How to Cite
Rho, Jong M., Anderson, Gail D., Donevan, Sean D. and White, H. Steve. (2002), Acetoacetate, Acetone, and Dibenzylamine (a Contaminant in l-(+)-β-Hydroxybutyrate) Exhibit Direct Anticonvulsant Actions in Vivo. Epilepsia, 43: 358–361. doi: 10.1046/j.1528-1157.2002.47901.x
- Issue published online: 22 APR 2002
- Article first published online: 22 APR 2002
- Revision accepted January 25, 2002.
- Acetoacetic acid;
- 3-Hydroxybutanoic acid;
- Ketogenic diet;
Summary: Purpose: To investigate whether ketone bodies are directly anticonvulsant.
Methods: We tested the effects of acetoacetate (ACA), acetone, and both stereoisomers, d-(−)- and l-(+), of β-hydroxybutyrate (BHB) on sensory-evoked seizures in Frings audiogenic seizure-susceptible mice.
Results: We found that these ketone bodies, with the exception of the d-(−)-isomer of BHB, were anticonvulsant in this model. Furthermore, with gas chromatography–mass spectrometry, we confirmed that the activity of l-(+)-BHB was due to dibenzylamine, a chemical contaminant.
Conclusions: Our data indicate that the anticonvulsant efficacy of the ketogenic diet may be due in part to the direct actions of ACA and acetone.