Prosaposin-derived peptides enhanced sprouting of sensory neurons in vitro and induced sprouting at motor endplates in vivo
Article first published online: 25 DEC 2001
Blackwell Science, Inc.
Journal of the Peripheral Nervous System
Volume 5, Issue 3, pages 126–130, September 2000
How to Cite
Campana, W. M., Mohiuddin, L., Misasi, R., O'brien, J.S. and Calcutt, N.A. (2000), Prosaposin-derived peptides enhanced sprouting of sensory neurons in vitro and induced sprouting at motor endplates in vivo. Journal of the Peripheral Nervous System, 5: 126–130. doi: 10.1046/j.1529-8027.2000.00019.x
- Issue published online: 25 DEC 2001
- Article first published online: 25 DEC 2001
- peripheral nerve;
- trophic factor;
Abstract Prosaposin exhibits neurotrophic factor properties that are localized to a 12-amino acid sequence located in the amino terminal portion of the saposin C domain. Prosaptides are peptides derived from the neurotrophic portion of prosaposin; these have been previously reported to be bioactive in neuroblastoma cell lines in vitro. We report that prosaptides were also bioactive in explants of adult primary sensory neurons by dose-dependently increasing both the number (3- to 4-fold) and elongation of these neurites by 50%. Local injection of prosaptides into the gluteus muscle of adult mice also induced sprouting at the motor endplate. Our results indicate that prosaptides are potent neuritogenic factors for both sensory and motor neurons of adult peripheral nerve.