Present address: Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA 24060, USA
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MORPHOLOGICAL AND 16S rRNA GENE EVIDENCE FOR RECLASSIFICATION OF THE PARALYTIC SHELLFISH TOXIN PRODUCING APHANIZOMENON FLOS-AQUAE LMECYA 31 AS APHANIZOMENON ISSATSCHENKOI (CYANOPHYCEAE)†
Article first published online: 28 JUL 2003
DOI: 10.1046/j.1529-8817.2003.02199.x
Additional Information
How to Cite
Li, R., Carmichael, W. W. and Pereira, P. (2003), MORPHOLOGICAL AND 16S rRNA GENE EVIDENCE FOR RECLASSIFICATION OF THE PARALYTIC SHELLFISH TOXIN PRODUCING APHANIZOMENON FLOS-AQUAE LMECYA 31 AS APHANIZOMENON ISSATSCHENKOI (CYANOPHYCEAE). Journal of Phycology, 39: 814–818. doi: 10.1046/j.1529-8817.2003.02199.x
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Received 27 December 2002. Accepted 27 April 2003.
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Present address: Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA 24060, USA
Publication History
- Issue published online: 28 JUL 2003
- Article first published online: 28 JUL 2003
- Abstract
- Article
- References
- Cited By
Keywords:
- Aphanizomenon issatschenkoi;
- cyanotoxin;
- 16S rDNA;
- morphology;
- gene sequences;
- taxonomy
A taxonomic reevaluation of the paralytic shellfish toxin (saxitoxins) producing cyanobacterium Aphanizomenon flos-aquae Ralfs ex Born. & Flah. LMECYA31 was done using morphology and 16S rRNA gene sequences. We found that strain LMECYA31 was incorrectly identified as Aph. flos-aquae based on (a) lack of bundle formation in trichomes, (b) shape of terminal cells in the trichomes, (c) lower similarity (<97.5%) in the 16S rRNA gene sequences relative to those of Aph. flos-aquae, and (d) comparison within a phylogenetic tree of 16S rRNA gene sequences. The shape of the terminal trichome cells and the shape and size of the vegetative cell, heterocyst, and akinete in strain LMECYA31 match characters of Aph. issatschenkoi (Ussachew) Proschkina-Larvernko. 16S rRNA gene sequences and phylogenetic clusters constructed from 16S rRNA gene sequences support our conclusion that strain LMECYA31 should be Aph. issatschenkoi.