Zenaida Ferrer, ART, National Reference Laboratory, Canadian Red Cross Blood, Transfusion Service, 95 Wellesley Street East, Toronot, Ontario, Canada, M4Y 1H6.
Comparison of a modified manual hexadimethrine bromide (Polybrene) and a low-ionic-strength solution antibody detection technique
Article first published online: 5 MAR 2003
Volume 25, Issue 2, pages 145–148, March-April 1985
How to Cite
Ferrer, Z., Wright, J., Moore, B. P.L. and Freedman, J. (1985), Comparison of a modified manual hexadimethrine bromide (Polybrene) and a low-ionic-strength solution antibody detection technique. Transfusion, 25: 145–148. doi: 10.1046/j.1537-2995.1985.25285169208.x
- Issue published online: 5 MAR 2003
- Article first published online: 5 MAR 2003
- Revision received August 20, 1984 and accepted September 10, 1984.
Manual hexadimethrine bromide (Polybrene) tests (Polybrene in low-ionic medium) were used in parallel with manual low-ionic-strength solution (LISS) procedures for the routine testing of patient samples referred to a general hospital blood bank. Of 5646 consecutive sera tested, 5167 (91.5%) did not react with either technique; 320 sera (5.7%) reacted in both methods. The Polybrene technique detected 63 antibodies which did not react in the LISS methods. One hundred sera did not react in the Polybrene test, but did react in the LISS methods. Sera showing discrepant results between the two methods were further tested in a reference laboratory. Polybrene tests appeared to be better in avoiding reactions due to clinically nonsignificant antibodies. The LISS methods, however, appeared to be more sensitive in detecting antibodies of potential clinical significance.