Hendrik C. van Prooijen, MD, Department of Immunohaematology, University Hospital Utrecht.
Acquired secretion defect in platelets after cryopreservation in dimethyl sulfoxide
Article first published online: 5 MAR 2003
Volume 26, Issue 4, pages 358–363, July-August 1986
How to Cite
Van Prooijen, H. C., Van Heugten, J. G., Mommersteeg, M. E. and Akkerman, J. W. N. (1986), Acquired secretion defect in platelets after cryopreservation in dimethyl sulfoxide. Transfusion, 26: 358–363. doi: 10.1046/j.1537-2995.1986.26486262744.x
- Issue published online: 5 MAR 2003
- Article first published online: 5 MAR 2003
- Received for publication May 1, 1985, and accepted July 9,1985.
Despite the use of preservatives, platelets are severely damaged during cryopreservation and, following freezing, function poorly in a number of in vitro tests. We report here that cryopreserved platelets show diminished aggregation in response to collagen. This may be a consequence of a secretion defect as evidenced by a 20 to 30 percent loss of dense- and α-granule content (p < 0.05) and an impaired secretion mechanism. Analysis of adenine nucleotides confirmed the defect in dense granule adenosine triphosphate (ATP) and adenosine diphosphate (ADP) content (storage pool), but in addition revealed a 50 percent fall in cytosolic ATP (metabolic pool). In contrast, the adenylate energy charge, (ATP + 1/2 ADP)/(ATP + ADP + adenosine monophosphate), was normal. We concluded that platelet cryopreservation leads to a secretion defect, probably as a result of activation during freezing and thawing procedures.