Shirley P. Levine, MD, Associate Professor, University of Texas Health Science Center.
A comparison of frozen and fresh platelet concentrates in the support of thrombocytopenic patients
Version of Record online: 5 MAR 2003
Volume 26, Issue 6, pages 525–530, November-December 1986
How to Cite
Towell, B. L., Levine, S. P., Knight, W. A. and Anderson, J. L. (1986), A comparison of frozen and fresh platelet concentrates in the support of thrombocytopenic patients. Transfusion, 26: 525–530. doi: 10.1046/j.1537-2995.1986.26687043618.x
- Issue online: 5 MAR 2003
- Version of Record online: 5 MAR 2003
- Received for publication September 4, 1985; revision received December 5, 1985, and accepted December 10, 1985.
Ten patients scheduled to receive intensive chemotherapy were plateletapheresed and the platelet-rich plasma was frozen with 5 percent dimethyl sulfoxide at –80 to –95° C until needed. Paired comparisons of frozen autologous platelets with fresh single-donor platelets were made in seven patients using corrected platelet increments at 1 and 24 hours, and pre- and posttransfusion bleeding times. In vitro tests of 12 units of plateletrich plasma before and after freezing included platelet factor 4 (PF4) secretion, malondialdehyde production, and electron microscopic evaluation of morphology. Fresh platelets provided significantly better 1- and 24-hour corrected increments compared with frozen autologous platelets. In only one case of alloimmunization did frozen autologous platelets provide a better increment than fresh platelets. Bleeding times after transfusion showed no consistent improvement regardless of type of transfusion or platelet count. Secretable PF4 remained constant after freezing, but malondialdehyde production fell significantly. Platelets showed considerable structural damage with 33 percent balloon forms counted after thawing, compared to less than 1 percent before freezing. Except in the case of alloimmunization, frozen autologous platelets are inferior to single-donor fresh platelets, and are significantly damaged in the freezing process.