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Extension of the maximum holding time for whole blood collected into a CPD-ADSOL system from 6 to 8 hours at ambient temperature under conditions that cause the temperature of the blood to decrease to 20 to 24° C would facilitate the preparation of platelet concentrates (PCs). In this study, the properties of CPD-PCs prepared and stored for 5 days in PL-732 containers after various initial holding periods were assessed in two laboratories, designated Laboratory A and Laboratory B. Laboratory A found higher platelet-rich plasma (PRP) volumes (276 ± 25 vs. 249 ± 19 mL) and platelet yields (76 ± 18 vs. 66 ± 18 × 109 platelets) with 8-hour holds (n = 10) than with 1- to 2-hour holds (n = 10), although only the difference in PRP volumes was significant (p < 0.05). No significant difference was observed in autologous in vivo recovery (54 ± 11 vs. 47 ± 9%) or survival (167 ± 37 vs. 170 ± 25 hrs), as calculated by gamma function using 111In as radiolabel. Laboratory B also found higher PRP volumes (304 ± 31 vs. 279 ± 37 mL) and platelet yields with 8-hour holds (n = 12) than with a 6-hour holds (n = 10) (88 ± 26 vs. 77 ± 27 × 109 platelets). No significant differences were found in morphology score, the extent of release of β-thromboglobulin, the discharge of lactate dehydrogenase, or hypotonic shock response. These studies showed that an 8-hour preprocessing hold period did not significantly affect in vitro and in vivo properties of PCs after 5 days of storage.