Martin Bruce, FIMLS, Reagents Manager, SNBTS Headquarters Unit Laboratory.
The preservation of red cell antigens at low ionic strength
Article first published online: 5 MAR 2003
Volume 30, Issue 5, pages 423–426, June 1990
How to Cite
Allan, J.C., Bruce, M. and Mitchell, R. (1990), The preservation of red cell antigens at low ionic strength. Transfusion, 30: 423–426. doi: 10.1046/j.1537-2995.1990.30590296374.x
- Issue published online: 5 MAR 2003
- Article first published online: 5 MAR 2003
- Received for publication April 3, 1989; revision received November 10, 1989, and accepted November 20, 1989
Low-ionic-strength saline (LISS) techniques permit a safe and substantial reduction in incubation time and have therefore become the method of choice for antibody detection and compatibility testing in many transfusion laboratories. Consequently, the supply of reagent red cells (RBCs) in a low-ionic-strength preservative solution would remove the daily need for laboratories to wash and resuspend cells in LISS before use. However, the storage of fresh RBCs at low ionic strength in the presence of aminoglycoside antibiotics can cause a rapid loss of certain antigens, possibly as a result of the release of proteolytic enzymes from contaminating white cells. This article describes a low-ionic-strength solution that achieves preservation of antigens on liquid nitrogen-frozen-thawed RBCs for 21 days' storage at 4°C.