Collection of WBC-reduced single-donor PLT concentrates with a new blood cell separator: results of a multicenter study

Authors

  • Roger Moog,

    Corresponding author
    1. From the Institute for Transfusion Medicine, University Clinics, Essen; the Institute for Transfusion Medicine and Hemostaseology, University Hospital, Marburg; the Department of Transfusion Medicine, Medical School, Hannover; the Department for Clinical Hemostaseology and Transfusion Medicine, University Clinics, Homburg; Aix Scientifics, Aachen; and the Department of Transfusion Medicine and Hemostaseology, University Clinics, Erlangen/Nürnberg, Germany.
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  • Thomas Zeiler,

    1. From the Institute for Transfusion Medicine, University Clinics, Essen; the Institute for Transfusion Medicine and Hemostaseology, University Hospital, Marburg; the Department of Transfusion Medicine, Medical School, Hannover; the Department for Clinical Hemostaseology and Transfusion Medicine, University Clinics, Homburg; Aix Scientifics, Aachen; and the Department of Transfusion Medicine and Hemostaseology, University Clinics, Erlangen/Nürnberg, Germany.
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  • Hans-Gert Heuft,

    1. From the Institute for Transfusion Medicine, University Clinics, Essen; the Institute for Transfusion Medicine and Hemostaseology, University Hospital, Marburg; the Department of Transfusion Medicine, Medical School, Hannover; the Department for Clinical Hemostaseology and Transfusion Medicine, University Clinics, Homburg; Aix Scientifics, Aachen; and the Department of Transfusion Medicine and Hemostaseology, University Clinics, Erlangen/Nürnberg, Germany.
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  • Berckard Stephan,

    1. From the Institute for Transfusion Medicine, University Clinics, Essen; the Institute for Transfusion Medicine and Hemostaseology, University Hospital, Marburg; the Department of Transfusion Medicine, Medical School, Hannover; the Department for Clinical Hemostaseology and Transfusion Medicine, University Clinics, Homburg; Aix Scientifics, Aachen; and the Department of Transfusion Medicine and Hemostaseology, University Clinics, Erlangen/Nürnberg, Germany.
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  • Eike G. Fischer,

    1. From the Institute for Transfusion Medicine, University Clinics, Essen; the Institute for Transfusion Medicine and Hemostaseology, University Hospital, Marburg; the Department of Transfusion Medicine, Medical School, Hannover; the Department for Clinical Hemostaseology and Transfusion Medicine, University Clinics, Homburg; Aix Scientifics, Aachen; and the Department of Transfusion Medicine and Hemostaseology, University Clinics, Erlangen/Nürnberg, Germany.
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  • Volker Kretschmer,

    1. From the Institute for Transfusion Medicine, University Clinics, Essen; the Institute for Transfusion Medicine and Hemostaseology, University Hospital, Marburg; the Department of Transfusion Medicine, Medical School, Hannover; the Department for Clinical Hemostaseology and Transfusion Medicine, University Clinics, Homburg; Aix Scientifics, Aachen; and the Department of Transfusion Medicine and Hemostaseology, University Clinics, Erlangen/Nürnberg, Germany.
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  • Regina Rödel-Spieker,

    1. From the Institute for Transfusion Medicine, University Clinics, Essen; the Institute for Transfusion Medicine and Hemostaseology, University Hospital, Marburg; the Department of Transfusion Medicine, Medical School, Hannover; the Department for Clinical Hemostaseology and Transfusion Medicine, University Clinics, Homburg; Aix Scientifics, Aachen; and the Department of Transfusion Medicine and Hemostaseology, University Clinics, Erlangen/Nürnberg, Germany.
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  • Stephan Strasser,

    1. From the Institute for Transfusion Medicine, University Clinics, Essen; the Institute for Transfusion Medicine and Hemostaseology, University Hospital, Marburg; the Department of Transfusion Medicine, Medical School, Hannover; the Department for Clinical Hemostaseology and Transfusion Medicine, University Clinics, Homburg; Aix Scientifics, Aachen; and the Department of Transfusion Medicine and Hemostaseology, University Clinics, Erlangen/Nürnberg, Germany.
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  • Jürgen Zingsem

    1. From the Institute for Transfusion Medicine, University Clinics, Essen; the Institute for Transfusion Medicine and Hemostaseology, University Hospital, Marburg; the Department of Transfusion Medicine, Medical School, Hannover; the Department for Clinical Hemostaseology and Transfusion Medicine, University Clinics, Homburg; Aix Scientifics, Aachen; and the Department of Transfusion Medicine and Hemostaseology, University Clinics, Erlangen/Nürnberg, Germany.
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  • Supported by Fresenius Hemocare (Bad Homburg, Germany).

Address reprint requests to: Rainer Moog, MD, PhD, Institute for Transfusion Medicine, University Clinics Essen, Hufelandstrasse 55, D-45122 Essen, Germany; e-mail: rainer.moog@uni-essen.de.

Abstract

BACKGROUND: A new cell separator (COM.TEC, Fresenius) was recently developed aimed at efficient collection of WBC-reduced single-donor PLT concentrates (SDPs).

STUDY DESIGN AND METHODS: Five German centers collected 554 WBC-reduced SDPs with help of the COM.TEC cell separator. Two multicenter cell counting studies were performed at the beginning and at the end of the study to document uniform counting results among the participating centers.

RESULTS: A total of 441 (79.6%) PLT collections were included in the study according to the protocol. A total of 342 single-dose and 99 double-dose SDPs were collected. For single-dose SDPs, an average blood volume of 2826 ± 409 mL was processed in a donation time of 55 ± 11 minutes. Mean PLT yield of these products was 3.11 × 1011± 0.40 × 1011 and the WBC contamination was 0.11 × 106± 0.20 × 106. For double-dose SDPs (PLT count, 5.29 ± 0.93 × 1011), 3943 ± 639 mL was processed. The average difference between the target and the collected PLT concentration was −2.8 ± 12.0 percent for single-dose SDPs and −1.8 ± 9.5 for double-dose SDPs, respectively. The collection efficiency was 53.7 ± 5.8 percent for single-dose SDPs and 58.2 ± 6.2 percent for double-dose SDPs. If all results of each sample from the counting study were set to unity (to the mean over all centers), most PLT determinations were very similar to the mean, for example, near or 1 if set to unity.

CONCLUSION: The COM.TEC machine makes it possible to obtain WBC-reduced SDPs that comply with current standards.

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