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Comparison of two apheresis systems for the collection of CD14+ cells intended to be used in dendritic cell culture

Authors

  • Erwin F. Strasser,

    Corresponding author
    1. From the Department of Transfusion Medicine and Haemostaseology, University Hospital, FAU Erlangen-Nürnberg; and the Department of Dermatology, University Hospital, FAU Erlangen-Nürnberg, Germany.
      Address reprint requests to: Erwin Strasser, MD, Department of Transfusion Medicine and Haemostaseology, University Hospital of Erlangen-Nürnberg, Krankenhausstr. 12, D-91054 Erlangen, Germany; e-mail: erwin.strasser@trans.imed.uni-erlangen.de.
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  • Thomas G. Berger,

    1. From the Department of Transfusion Medicine and Haemostaseology, University Hospital, FAU Erlangen-Nürnberg; and the Department of Dermatology, University Hospital, FAU Erlangen-Nürnberg, Germany.
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  • Volker Weisbach,

    1. From the Department of Transfusion Medicine and Haemostaseology, University Hospital, FAU Erlangen-Nürnberg; and the Department of Dermatology, University Hospital, FAU Erlangen-Nürnberg, Germany.
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  • Robert Zimmermann,

    1. From the Department of Transfusion Medicine and Haemostaseology, University Hospital, FAU Erlangen-Nürnberg; and the Department of Dermatology, University Hospital, FAU Erlangen-Nürnberg, Germany.
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  • Jürgen Ringwald,

    1. From the Department of Transfusion Medicine and Haemostaseology, University Hospital, FAU Erlangen-Nürnberg; and the Department of Dermatology, University Hospital, FAU Erlangen-Nürnberg, Germany.
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  • Beatrice Schuler-Thurner,

    1. From the Department of Transfusion Medicine and Haemostaseology, University Hospital, FAU Erlangen-Nürnberg; and the Department of Dermatology, University Hospital, FAU Erlangen-Nürnberg, Germany.
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  • Jürgen Zingsem,

    1. From the Department of Transfusion Medicine and Haemostaseology, University Hospital, FAU Erlangen-Nürnberg; and the Department of Dermatology, University Hospital, FAU Erlangen-Nürnberg, Germany.
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  • Reinhold Eckstein

    1. From the Department of Transfusion Medicine and Haemostaseology, University Hospital, FAU Erlangen-Nürnberg; and the Department of Dermatology, University Hospital, FAU Erlangen-Nürnberg, Germany.
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  • This work was supported in part by a grant from the German Research Ministry (BMBF), Bonn, Germany, grant number 01 GE 9601 and the ELAN-Program (“Fond fuer Forschung und Lehre”) of the University Erlangen-Nuremberg, Germany.

  • TRANSFUSION 2003;43:1309-1316.

Address reprint requests to: Erwin Strasser, MD, Department of Transfusion Medicine and Haemostaseology, University Hospital of Erlangen-Nürnberg, Krankenhausstr. 12, D-91054 Erlangen, Germany; e-mail: erwin.strasser@trans.imed.uni-erlangen.de.

Abstract

BACKGROUND:  Monocytes collected by leukapheresis are increasingly used for dendritic cell (DC) culture in cell factories suitable for DC vaccination in cancer.

STUDY DESIGN AND METHODS:  Using modified MNC programs on two apheresis systems (Cobe Spectra and Fresenius AS.TEC204), leukapheresis components collected from 84 patients with metastatic malignant melanoma and from 31 healthy male donors were investigated. MNCs, monocytes, RBCs, and platelets (PLTs) in donors and components were analyzed by cell counters, WBC differential counts, and flow cytometry.

RESULTS:  In 5-L collections, Astec showed better results regarding monocyte collection rates (11.0 vs. 7.4 × 106/min, p = 0.04) and efficiencies (collection efficiency, 51.9 vs. 31.9%; p < 0.001). Both devices resulted in monocyte yields at an average of 1 × 109 (donors) and 2.5 × 109 (patients), whereas Astec components contained high residual RBCs. Compared to components with low residual PLTs, high PLT concentration resulted in higher monocyte loss (48 vs. 20%, p < 0.0001) before DC culture.

CONCLUSION:  The Astec is more efficient in 5-L MNC collections compared to the Spectra. Components with high residual PLTs result in high MNC loss by purification procedures. Thus, optimizing MNC programs is essential to obtain components with high MNC yields and low residual cells as prerequisite for high DC yields.

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