Supported in part by enCyte Systems, Inc.
Effects of rejuvenation and frozen storage on 42-day-old AS-3 RBCs
Version of Record online: 24 OCT 2003
Volume 43, Issue 11, pages 1527–1532, November 2003
How to Cite
Lockwood, W. B., Hudgens, R. W., Szymanski, I. O., Teno, R. A. and Gray, A. D. (2003), Effects of rejuvenation and frozen storage on 42-day-old AS-3 RBCs. Transfusion, 43: 1527–1532. doi: 10.1046/j.1537-2995.2003.00551.x
- Issue online: 24 OCT 2003
- Version of Record online: 24 OCT 2003
- Received for publication March 10, 2003; revision received June 20, 2003, and accepted June 23, 2001.
BACKGROUND: A storage period of 42 days has been approved by the FDA for RBCs stored in NUTRICEL (AS-3). This study was undertaken to provide data to the FDA about the feasibility of salvaging AS-3 RBCs at the end of their storage period by rejuvenation and freezing, and to evaluate the effect of rejuvenation on indicators of RBC function.
STUDY DESIGN AND METHODS: Healthy adults (n = 22) donated 450 mL of whole blood, and RBC components were prepared at two study sites. The components were stored at 1 to 6°C for either 41 days (nonrejuvenated frozen controls, n = 6) or 42 days (rejuvenated frozen study group, n = 10; nonrejuvenated nonfrozen controls, n = 6). Rejuvenated study components and nonrejuvenated frozen controls were stored at −70°C for longer than 2 weeks. Frozen units were then deglycerolized and kept for an additional 24 hours at 1 to 6°C.
RESULTS: 2,3-DPG, and ATP were reduced after 42-day storage to near 0 and 65 percent, respectively, of their original values. After rejuvenation and deglycerolization, the mean ATP level was 146 percent and the mean 2,3-DPG was 115 percent. The percent freeze-thaw-wash recovery was similar for rejuvenated and nonrejuvenated RBCs. Trace amounts of hypoxanthine and inosine were detected in rejuvenated units. The mean 24-hour survival (single- or double-label technique) of all components exceeded 75 percent. The t1/2 of study and control RBCs was similar.
CONCLUSION: The ability of 42-day-old AS-3 RBCs to deliver oxygen after rejuvenation and freezing is not impaired. These data indicate that rejuvenated AS-3 RBCs can provide a safe and beneficial blood component immediately upon infusion.