A Digital Method of Sperm Immobilization Test: Comparison to the Conventional Method
Version of Record online: 5 NOV 2003
American Journal of Reproductive Immunology
Volume 50, Issue 6, pages 481–484, December 2003
How to Cite
Komori, S., Hamada, Y., Hasegawa, A., Tsubamoto, H., Horiuchi, I., Tanaka, H., Kasumi, H., Shigeta, M. and Koyama, K. (2003), A Digital Method of Sperm Immobilization Test: Comparison to the Conventional Method. American Journal of Reproductive Immunology, 50: 481–484. doi: 10.1046/j.8755-8920.2003.00108.x
- Issue online: 5 NOV 2003
- Version of Record online: 5 NOV 2003
- Submitted January 31, 2003; revised May 21, 2003; accepted May 30, 2003.
- antisperm antibody;
- sperm immobilizing antibody;
- Sperm immobilization test;
- Sperm Quality Analyzer
Antisperm antibodies have been found in infertile patients and those causing immobilization of sperm are considered to be closely related to unexplained infertility. These antibodies are usually identified by a sperm immobilization test which involves counting motile sperm under microscope. This test is subjective as it relies on the judgement of the examiner with respect to sperm motility. In this study, we analyzed motile sperm by a digital method using Sperm Quality Analyzer. The results were compared with those obtained by the conventional method. We found that the two methods yielded identical results, with 14 of 66 samples tested being positive and 52 negative for sperm immobilizing antibodies. These results show that the digital method is objective and of value in the measurement of motile sperm in determination of sperm immobilizing antibodies.