NF-κB Activation at Implantation Window of the Mouse Uterus
Article first published online: 16 JAN 2004
American Journal of Reproductive Immunology
Volume 51, Issue 1, pages 16–21, January 2004
How to Cite
Nakamura, H., Kimura, T., Ogita, K., Nakamura, T., Takemura, M., Shimoya, K., Koyama, S., Tsujie, T., Koyama, M. and Murata, Y. (2004), NF-κB Activation at Implantation Window of the Mouse Uterus. American Journal of Reproductive Immunology, 51: 16–21. doi: 10.1046/j.8755-8920.2003.00116.x
- Issue published online: 16 JAN 2004
- Article first published online: 16 JAN 2004
- Submitted February 19, 2003; revised July 15, 2003; accepted July 17, 2003.
- implantation window;
- nuclear factor kappa B
Problem: Nuclear factor kappa B (NF-κB) is one candidate transcriptional modulator, which might regulate many kinds of molecules that play sequential roles at implantation in the endometrium. However, temporal and spatial activation of NF-κB at implantation window is unknown.
Methods: Activation of NF-κB in the mouse uterus was determined by electrophoretic mobility shift assays. Localization of p50 and p65, components of NF-κB, was analyzed by immunohistochemistry.
Results: NF-κB was activated in the proestrus and estrus phases in non-pregnant uterus. In the pregnant uterus, NF-κB was activated after day 1.5 post-coitum, and the activation continued during implantation period. The immunoreactivities of p50 and p65 were mainly localized in endometrial epithelium, and were weaker in endometrial stroma cells.
Conclusion: NF-κB activity is dynamically regulated during the sexual cycle as well as during the implantation period in the endometrium, where the biochemical interaction between mother and conceptus first occurs.