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NF-κB Activation at Implantation Window of the Mouse Uterus

Authors

  • Hitomi Nakamura,

    1. Division of Obstetrics and Gynecology, Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, Osaka, Japan
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  • Tadashi Kimura,

    1. Division of Obstetrics and Gynecology, Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, Osaka, Japan
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  • Kazuhide Ogita,

    1. Division of Obstetrics and Gynecology, Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, Osaka, Japan
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  • Takafumi Nakamura,

    1. Department of Obstetrics and Gynecology, Toyama Medical and Pharmaceutical University, Toyama, Japan
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  • Masahiko Takemura,

    1. Division of Obstetrics and Gynecology, Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, Osaka, Japan
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  • Koichiro Shimoya,

    1. Division of Obstetrics and Gynecology, Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, Osaka, Japan
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  • Sinsuke Koyama,

    1. Division of Obstetrics and Gynecology, Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, Osaka, Japan
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  • Tomoko Tsujie,

    1. Division of Obstetrics and Gynecology, Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, Osaka, Japan
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  • Masayasu Koyama,

    1. Division of Obstetrics and Gynecology, Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, Osaka, Japan
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  • Yuji Murata

    1. Division of Obstetrics and Gynecology, Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, Osaka, Japan
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Address reprint requests to Tadashi Kimura, Division of Obstetrics and Gynecology, Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 5650871, Japan.
E-mail: tadashi@gyne.med.osaka-u.ac.jp

Abstract

Problem:  Nuclear factor kappa B (NF-κB) is one candidate transcriptional modulator, which might regulate many kinds of molecules that play sequential roles at implantation in the endometrium. However, temporal and spatial activation of NF-κB at implantation window is unknown.

Methods:  Activation of NF-κB in the mouse uterus was determined by electrophoretic mobility shift assays. Localization of p50 and p65, components of NF-κB, was analyzed by immunohistochemistry.

Results:  NF-κB was activated in the proestrus and estrus phases in non-pregnant uterus. In the pregnant uterus, NF-κB was activated after day 1.5 post-coitum, and the activation continued during implantation period. The immunoreactivities of p50 and p65 were mainly localized in endometrial epithelium, and were weaker in endometrial stroma cells.

Conclusion:  NF-κB activity is dynamically regulated during the sexual cycle as well as during the implantation period in the endometrium, where the biochemical interaction between mother and conceptus first occurs.

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