Expression of IL-4, IL-8 and IL-18 Messenger RNAs in Maternal Peripheral Blood and Relationships with the HbF-γ chain mRNA in it

Authors

  • Chikako Kato,

    1. Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kawaramachi Hirokouji, Kamigyo-Ku, Kyoto, Japan
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  • Takaya Tamura,

    1. Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kawaramachi Hirokouji, Kamigyo-Ku, Kyoto, Japan
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  • Tomohiro Okuda,

    1. Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kawaramachi Hirokouji, Kamigyo-Ku, Kyoto, Japan
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  • Hidenori Kojima,

    1. Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kawaramachi Hirokouji, Kamigyo-Ku, Kyoto, Japan
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  • Yoshiyuki Kinoshita,

    1. Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kawaramachi Hirokouji, Kamigyo-Ku, Kyoto, Japan
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  • Hideo Honjo

    1. Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kawaramachi Hirokouji, Kamigyo-Ku, Kyoto, Japan
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Address reprint requests to Chikako Kato, Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, 465 Kajii-Cho, Kawaramachi Hirokouji, Kamigyo-Ku, Kyoto 602-8566, Japan.
E-mail: ckato@koto.kpu-m.ac.jp

Abstract

Problem:  This study was designed to examine immunological changes in maternal peripheral blood and the relationship of these changes with the amount of fetal cells in the blood.

Method of study:  The expression of interleukin-4 (IL-4), IL-8, IL-18 and fetal hemoglobin gamma chain (HbF-γ chain) messenger RNAs (mRNAs) in maternal peripheral blood was measured by a quantitative reverse transcription-polymerase chain reaction method.

Results:  In maternal peripheral blood, the expression of IL-4 mRNA was up-regulated from the second gestational month (GM) to delivery. The expression of IL-8 and IL-18 mRNAs was down-regulated from the third or fourth GM until the eighth or ninth GM, respectively, and both increased before the onset of labor, though IL-4 mRNA decreased. The expression of IL-8 and IL-18, but not IL-4, mRNAs was correlated with that of HbF-γ chain mRNA.

Conclusions:  Immunological interactions between maternal peripheral immune cells and fetal cells appear to be related to the onset of labor.

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