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Therapy with Dendritic Cells Influences the Spontaneous Resorption Rate in the CBA/J × DBA/2J Mouse Model

Authors

  • Sandra Blois,

    1. IDEHU, Institute of Humoral Immunity Studies, National Council of Scientific and Technological Research (CONICET), University of Buenos Aires, Buenos Aires, Argentina
    2. Charité, Department of Internal Medicine, Biomedizinisches Forschungszentrum, Campus Virchow, Humboldt University of Berlin, Berlin, Germany
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  • Catalina D. Alba Soto,

    1. Department of Microbiology, Parasitology and Immunology, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina
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  • Sofia Olmos,

    1. IDEHU, Institute of Humoral Immunity Studies, National Council of Scientific and Technological Research (CONICET), University of Buenos Aires, Buenos Aires, Argentina
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  • Eduardo Chuluyan,

    1. Immunogenetics Division, Hospital de Clínicas, Buenos Aires, Argentina
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  • Teresa Gentile,

    1. IDEHU, Institute of Humoral Immunity Studies, National Council of Scientific and Technological Research (CONICET), University of Buenos Aires, Buenos Aires, Argentina
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  • Petra C. Arck,

    1. Charité, Department of Internal Medicine, Biomedizinisches Forschungszentrum, Campus Virchow, Humboldt University of Berlin, Berlin, Germany
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  • Ricardo A. Margni

    1. IDEHU, Institute of Humoral Immunity Studies, National Council of Scientific and Technological Research (CONICET), University of Buenos Aires, Buenos Aires, Argentina
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  • Presented at the 8th Congress of the Alps Adria Society for Immunology of Reproduction, Weimar, Germany, September 2002.

Address reprint requests to Sandra Blois, Biomedizinisches Forschungszentrum, Raum 2.0549, Campus Virchow, Humboldt University of Berlin, Augustenburger Platz 1, 13353 Berlin, Germany.
E-mail: sblois@ffyb.uba.ar

Abstract

Problem:  DBA/2J-mated CBA/J female mice are prone to a high incidence of fetal abortions. This fetal wastage can be dramatically reduced by immunizing the female mice with BALB/c, but not with DBA/2J spleen cells during early gestation. Nevertheless, the underlying mechanisms remain to be elucidated. Recently, dendritic cells (DC) have been described at the feto-maternal interface in the human uterus. In this work, we studied the effect of adoptive transfer of DC on the maintenance of pregnancy in the CBA/J × DBA/2J model.

Methods:  Bone marrow-derived DC were generated from virgin female CBA/J mice (6–8 weeks old). CBA/J females were inoculated with DC twice before mating. Four different experimental groups were included: (i) no treatment control, (ii) mice injected with culture medium [granulocyte–macrophage colony-stimulating factor (GM-CSF)], (iii) immunized with DC and (iv) immunized with paternal DBA/2J antigens lisate-pulsed DC, n = 5.

Results:  The control abortion rate was 23.8%, and with GM-CSF alone was 17.6%. Following inoculation of syngeneic DC abortion rates were reduced to 2.2%, but protection was short-lived. Abortion rates with DC pulsed with DBA/2J antigens was 5%. Serum of interleukin (IL)-6 levels were lower in the latter two groups up to the time of abortion. The kinetics of immunoglobulin G asymmetric antibodies synthesis was modified, but there was no correlation between asymmetric antibodies production and the lowering of abortions rates.

Conclusion:  Syngeneic DC prevented abortions and this was linked to a decrease in IL-6 levels, but not with levels of asymmetric antibodies.

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