Regulation of Interleukin-6 Fetoplacental Levels could be Involved in the Protective Effect of Low-molecular Weight Heparin Treatment on Murine Spontaneous Abortion


Address reprint requests to Gabriela Gutiérrez, IDEHU-Cátedra de Inmunología, Facultad de farmacia y Bioquímica, Universidad de Buenos Aires, Junin 956, Piso 4 (1113), Argentina.


Problem:  CBA/J × DBA/2 abortion rate could be the consequence of a deficient local production of T helper (Th2) cytokines, which cause fetal wastage via fgl2 prothrombinase. Heparin reduces significantly the abortion rate in mice and recurrent spontaneous abortion (RSA) patients. We proposed to determine the effect of enoxaparin on the levels of local interleukin (IL)-6 during murine pregnancy.

Method of study:  Recombinant human IL-6 (rhIL-6) or enoxaparin were inoculated in CBA/J × DBA/2 pregnant mice on days 6.5–12.5. IL-6 levels in sera as well as in culture supernatants of day 9.5 fetoplacental units of CBA/J × BALB/c control mice or CBA/J × DBA/2 abortion combination were determined by enzyme-linked immunosorbent assay (ELISA) test.

Results:  CBA/J × DBA/2 fetoplacental units secreted significantly lower levels of IL-6 with regard to CBA/J × BALB/c normal units. rhIL-6h and enoxaparin treatments decreased the resorption rate and regulated IL-6 fetoplacental levels.

Conclusion:  This study suggests that regulation of IL-6 fetoplacental levels could be involved in heparin-mediated anticoagulation protection against abortion.