Antiphosphatidylserine Antibodies Affect Rat Yolk Sacs in Culture: a Mechanism for Fetal Loss in Antiphospholipid Syndrome

Authors

  • Shelly Tartakover Matalon,

    1. Department of Anatomy and Cell Biology, Laboratory of Teratology, Hebrew University Hadassah Medical School, Jerusalem
    2. Department of Medicine ‘B’ and The Center for Autoimmune Diseases, Sheba Medical Center, Tel-Hashomer, and Sackler Faculty of Medicine, Tel-Aviv University
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  • Yehuda Shoenfeld,

    1. Department of Medicine ‘B’ and The Center for Autoimmune Diseases, Sheba Medical Center, Tel-Hashomer, and Sackler Faculty of Medicine, Tel-Aviv University
    2. Incumbent of the Laura Schwarz-Kipp Chair for Research of Autoimmune Diseases, Tel-Aviv University, Tel-Aviv, Israel
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  • Miri Blank,

    1. Department of Medicine ‘B’ and The Center for Autoimmune Diseases, Sheba Medical Center, Tel-Hashomer, and Sackler Faculty of Medicine, Tel-Aviv University
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  • Sarah Yacobi,

    1. Department of Anatomy and Cell Biology, Laboratory of Teratology, Hebrew University Hadassah Medical School, Jerusalem
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  • Philipp Von Landenberg,

    1. Institute of Clinical Chemistry and Laboratory Medicine, Johannes-Gutenberg-University, Mainz, Germany
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  • Asher Ornoy

    1. Department of Anatomy and Cell Biology, Laboratory of Teratology, Hebrew University Hadassah Medical School, Jerusalem
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Address reprint requests to Prof. Yehuda Shoenfeld, Department of Medicine ‘B’, Sheba Medical Center, Tel Hashomer 52621, Israel.
E-mail: shoenfel@post.tau.ac.il

Abstract

Problem:  A variety of reproductive impairments have been reported in the context of the antiphospholipid syndrome (APS). APS is associated with the presence of antibodies to negatively charged phospholipids that may affect the outcome of pregnancy.

Method of study:  Rat embryos were cultured within their yolk sacs. The effects of two antiphosphatidylserine monoclonal aPS antibodies (HL5B, RR7F) regarding their influence on growth and apoptotic events of the yolk sacs, as well as on growth and the morphology of the embryos, were studied.

Results:  Exposure of rat embryos and their yolk sacs to aPS inhibited yolk sac growth. Moreover, increased number of apoptotic events of giant cells in the aPS-exposed ectoplacental cone was found in comparison with control IgG-exposed giant cells (P < 0.05). No significant damage was observed in the embryos.

Conclusions:  The results suggest that aPS affect growth and apoptosis of rat ectoplacental cone.

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