• 1
    Boudreau N, Bissell M. Extracellular matrix signaling: integration of form and function in normal and malignant cells. Cur Opin Cell Biol 1998; 10:640646.
  • 2
    Yu AE, Hewitt RE, Kleiner DE, Stetler SW. Molecular regulation of cellular invasion-role of gelatinase A and TIMP-2. Biochem Cell Biol 1996; 74:823831.MEDLINE
  • 3
    Sato H, Takino T, Okada Y, Cao J, Shinagawa A, Yamamoto E, Seiki M. A matrix metalloproteinase expressed on the surface of invasive tumour cells. Nature 1994; 370:6165.MEDLINE
  • 4
    Strongin AY, Collier I, Bannikov G, Marmer L, Grant GA, Goldberg GI. Mechanism of cell surface activation of 72-kDa type IV collagenase. J Biol Chem 1995; 270:53315338.MEDLINE
  • 5
    Kinoshita T, Sato H, Okada A, Ohuchi E, Imai K, Okada Y, Seiki M. TIMP2 promotes activation of progelatinase A by membrane-type 1 matrix metalloproteinase immobilized on agarose beads. J Biol Chem 1998; 273:1608816103.
  • 6
    Akriviadis EA, Llovet JM, Efremidis SC, Shouval D, Canelo R, Ringe B, Meyers WC. Hepatocellular carcinoma. Br J Surg 1998; 85:13191331.MEDLINE
  • 7
    Johson PJ, Williams R. Cirrhosis and the aetiology of hepatocellular carcinoma. J Hepatol 1987; 4:140146.MEDLINE
  • 8
    Milani S, Herbst H, Schuppan D, Grappone C, Pelligrini G, Pinzani M, Casini A, et al. Differential expression of matrix metalloproteinase-1 and -2 genes in normal and fibrotic human liver. Am J Pathol 1994; 144:528537.MEDLINE
  • 9
    Benyon RC, Iredale JP, Goddard S, Winwood PJ, Arthur MJP. Expression of tissue inhibitor of metalloproteinases 1 and 2 is increased in fibrotic human liver. Gastroenterology 1996; 110:821831.MEDLINE
  • 10
    Yamamoto H, Itoh F, Adachi Y, Sakamoto H, Adachi M, Hinoda Y, Imai K. Relation of enhanced secretion of active matrix metalloproteinases with tumor spread in human hepatocellular carcinoma. Gastroenterology 1997; 112:12901296.MEDLINE
  • 11
    Harada T, Arii S, Mise M, Imamura T, Higashitsuji H, Furutani M, Niwano M, et al. Membrane-type matrix metalloproteinase-1 (MT1-MMP) gene is overexpressed in highly invasive hepatocellular carcinomas. J Hepatol 1998; 28:231239.MEDLINE
  • 12
    Musso O, Théret N, Campion JP, Turlin B, Milani S, Grappone C, Clément B. In Situ detection of matrix metalloproteinase-2 (MMP2) and the metalloproteinase inhibitor TIMP2 transcripts in human primary hepatocellular carcinoma and in liver metastasis. J Hepatol 1997; 26:593605MEDLINE
  • 13
    Clément B, Levavasseur F, Loréal O, Liétard J, L'Helgoualc'h A, Guillouzo A. Role of hepatocytes and Ito cells in the synthesis and deposition of extracellular matrix. Fat Storing Cells and Liver Fibrosis. Edited by SurrentiC, CasiniA, MilaniS, PinzaniM. Dordrecht: Kluwer Academic Publishers. 1992;1322.
  • 14
    Arthur MJP, Stanley A, Iredale JP, Rafferty JA, Hembry RM, Friedman SL. Secretion of 72-kDa type IV collagenase/gelatinase by cultured human lipocytes: Analysis of gene expression, protein synthesis and proteinase activity. Biochem J 1992; 287:701707.MEDLINE
  • 15
    Théret N, Musso O, L'Helgoualc'h A, Clément B. Activation of matrix metalloproteinase-2 from hepatic stellate cells requires interactions with hepatocytes. Am J Pathol 1997; 150:5158.MEDLINE
  • 16
    Théret N, Musso O, L'Helgoualc'h A, Campion JP, Clément B. Differential expression and origin of membrane-type 1 and 2 matrix metalloproteinase (MT1-MMPs) in association with MMP2 activation in injured human livers. Am J Pathol 1998; 153:945954.MEDLINE
  • 17
    Théret N, Lehti K, Musso O, Clément B. MMP2 activation by collagen I and concanavalin A in cultured human hepatic stellate cells. Hepatology 1999; 30:462468.MEDLINE
  • 18
    Azzam HS, Thompson EW. Collagen-induced activation of the Mt 72,000 type IV collagenase in normal and malignant human fibroblastoid cells. Cancer Res 1992; 52:45404544.MEDLINE
  • 19
    Seltzer JL, Lee AY, Akers KT, Sudbeck B, Southon EA, Wayner EA, Eisen AZ. Activation of 72-kDa type IV collagenase/gelatinase by normal fibroblasts in collagen lattices is mediated by integrin receptors but not related to lattice contraction. Exp Cell Res 1994; 213:365374.MEDLINE
  • 20
    Haas TL, Sandra JD, Madri JA. Three-dimensional type I collagen lattices induce coordinate expression of matrix metalloproteinases MT1-MMP and MMP-2 in microvascular endothelial cells. J Biol Chem 1998; 273:36043610.MEDLINE
  • 21
    Ellerbroek SM, Stack S. Membrane associated matrix metalloproteinase in metastasis. Bioassays 1999; 21:940949.
  • 22
    Edmonson H, Steiner P. Primary carcinoma of the liver: a study of 100 cases among 48900 necropsies. Cancer 1954; 7:462503.
  • 23
    Bedossa P, Poynard T. An algorithm for the grading of activity in chronic hepatitis C. Hepatology 1996; 24:289293.MEDLINE
  • 24
    Liétard J, Musso O, Theret N, L'Helgoualc'h A, Campion JP, Yamada Y, Clément B. SP1-mediated transactivation of LAMC1 promoter and coordinated expression of laminin γ1 and SP1 in human hepatocellular carcinomas. Am J Path 1997; 151:16631672.MEDLINE
  • 25
    Tromp G, Kuivaniemi H, Stacey A, Shikata H, Baldwin CT, Jaenisch R, Prockop DJ. Structure of a full-length cDNA clone for the prepro alpha 1(I) chain of human type I procollagen. Biochem J 1988; 253:919922.MEDLINE
  • 26
    Chan YL, Gutell R, Noller HF, Wool IG. The nucleotide sequence of a rat 18S ribosomal ribonucleic acid gene and a proposal for the secondary structure of 18S ribosomal ribonucleic acid. J Biol Chem 1984; 259:224230.MEDLINE
  • 27
    Musso O, Clément B, Theret N. 2000. In Methods in Molecular Medecine, vol 45: Hepatocellular Carcinoma Methods and Protocols, edited by N.A. Habib Humana Press, pp 139156.
  • 28
    Chomczynski P, Sacchi N. Single-step method of RNA isolation by guanidinium thiocyanate phenol chloroform extraction. Anal Biochem 1987; 162:156169.MEDLINE
  • 29
    Faouzi S, Le Bail B, Neaud V, Boussarie L, Saric J, Bioulac-Sage P, Balabaud C, et al. Myofibroblasts are responsible for collagen synthesis in the stroma of human hepatocellular carcinoma: an in vivo and in vitro study. J Hepatol 1999; 30:27584.MEDLINE
  • 30
    Kemeny F, Vadrot J, Wu A, Smadja C, Meakins JL, Franco D. Morphological and histological features of resected hepatocellular carcinoma in patients in the West. Hepatology 1989; 9:253257.MEDLINE
  • 31
    Lai ECS, Ng IOL, Ng MMT, Lok ASF, Tam PC, Fan ST, et al. Long-term results of resection for large hepatocellular carcinoma: a multivariate analysis of clinicalpathological features. Hepatology 1990; 11:815818.MEDLINE
  • 32
    Franco D, Capussotti L, Smadja C, Bouzari H, Meakins J, Kemeny F, Grange D, Dellepiane M. Resection of hepatocellular carcinomas: results in 72 European patients with cirrhosis. Gastroenterology 1993; 268:2501525018.
  • 33
    Nagasue N, Uchida M, Makino Y, Takemoto Y, Yamanoi A, Hayashi T, Chang YC, et al. Incidence and factors associated with recurrence following resection of hepatocellular carcinoma. Gastroenterology, 1993; 105:488494.
  • 34
    Ng IOL, Lai ECS, Ng MMT, Fan ST. Tumor encapsulation in hepatocellular carcinoma. A pathological study of 189 cases. Cancer 1992; 70:4549.MEDLINE
  • 35
    Kuriyama S, Yamazaki M, Mitoro A, Tsujimoto T, Okuda H, Tsujinoue H, Nakatami T, et al. Hepatocellular carcinoma in an orthotopic mouse model metastasizes intrahepatically in the cirrhotic liver but not in the normal liver. Hepatology 2000; 32:207A
  • 36
    Johnsen M, Lund LR, Romer J, Almholt K, Dano K. Cancer invasion and tissue remodeling: common themes in proteolytic matrix degradation. Curr Op Cell Biol 1998; 10:667671.MEDLINE
  • 37
    Bissell M. Hepatic fibrosis as wound repair: a progress report. J Gastroenterol 1998; 33:295302.MEDLINE
  • 38
    Othani H. Stromal reaction in cancer tissue: patholophysiologic significance of the expression of matrix-degrading enzymes in relation to matrix turnover and immune/inflammatory reactions. Pathol International 1998; 48:19.
  • 39
    Preaux AM, Mallat M, Van Nhieu JT, d' Ortho MP, Hembry R, Mavier P. Matrix metalloproteinase-2 activation in human hepatic fibrosis regulation by cell-matrix interaction. Hepatology 1999; 30:944950.MEDLINE
  • 40
    Siegel RC, Chen KH, Greenspan JS, Aguiar JM. Biochemical and immmunohistochemical study of lysyl oxidase in experimental hepatic fibrosis in the rat. Proc Natl Acad Sci U S A 1978; 75:29452949.MEDLINE
  • 41
    Peyrol S, Raccurt M, Gerard F, Gleyzal C, Grimaud JA, Sommer P. Lysyl oxidase gene expression in the stromal reaction to in situ and invasive ductal breast carcinoma. Am J Pathol 1997; 150:497507.MEDLINE
  • 42
    Stanton H, Gavrilovic J, Atkinson SJ, d'Ortho MP, Yamada KM, Zardi L, Murphy G. The activation of proMMP2 (gelatinase A) by HT1080 fibrosarcoma cells is promoted by culture on a fibronectin substrate and is concomitant with an increase in processing of MT1-MMP (MMP-14) to a 45kDa form. J Cell Sci 1998; 111:278998MEDLINE
  • 43
    Gilles C, Bassuk JA, Pulyaeva H, Sage EH, Foidart JM, Thompson EW. SPARC/osteonectin induces matrix metalloproteinase 2 activation in human breast cancer cell lines. Cancer Res 1998; 58:552936.MEDLINE
  • 44
    Torimura T, Ueno T, Inuzuka S, Kim M, Ohira H, Kimura Y, Majima Y, et al. The extracellular matrix in hepatocellular carcinoma show different localization patterns depending on the differentiation and the histological pattern of tumors. J Hepatol 1994; 21:3746.MEDLINE
  • 45
    Blendis L, Wong F, Sherman M. Interferon therapy prevents hepatocellular carcinomas in some patients with chronic HCV: the role of fibrosis. Gastroenterology 2000; 118:446448.MEDLINE
  • 46
    Clark EA, Golub TR, Lander ES, Hynes RO. Genomic analysis of metastasis reveals an essential role for RhoC. Nature 2000; 406:532535.MEDLINE
  • 47
    Volpes R, van den Oord JJ, Desmet VJ. Integrins as differential cell lineage markers of primary liver tumors. Am J Pathol 1993: 142:14831492.MEDLINE
  • 48
    Ivaska J, Heino J. Adhesion receptors and cell invasion: mechanisms of integrin-guided degradation of extracellular matrix. Cell Mol Life Sci 2000; 57:1624.MEDLINE