Correction of CFTR malfunction and stimulation of Ca2+-activated Cl channels restore HCOmath image secretion in cystic fibrosis bile ductular cells

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Abstract

In view of the occurrence of hepatobiliary disorders in cystic fibrosis (CF) this study addresses the role of the cystic fibrosis transmembrane conductance regulator (CFTR) and of Ca2+-activated Cl channels in promoting HCOmath image secretion in bile ductular cells. Human cholangiocytes were isolated from control livers and from 1 patient with CF (ΔF508/G542X mutations). Single channel and whole cell currents were analyzed by patch clamp techniques, and HCOmath image secretion was determined by fluorometric analysis of the rate of recovery of intracellular pH following alkaline loading. In control cholangiocytes, both cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) catalytic subunit, activated CFTR Cl channels that exhibited a nonrectifying conductance of 8 pS and appeared in clusters. Activation of Cl current by cAMP was associated with an increase in the rate of HCOmath image secretion. The basal rate of HCOmath image secretion was lower in CF than in control cholangiocytes. In both control and CF cholangiocytes, raising intracellular Ca2+ concentrations with ionomycin led to a parallel activation of Cl current and HCOmath image secretion. Consistent with reports that premature stop codon mutations (class I; e.g., G542X) can be read over by treatment with aminoglycoside antibiotics, exposure of CF cholangiocytes to gentamicin restored activation by cAMP of Cl current and HCOmath image secretion. The observation that activation of Ca2+-dependent Cl channels can substitute for cystic fibrosis transmembrane conductance regulator (CFTR) in supporting HCOmath image secretion and the efficacy of gentamicin in restoring CFTR function and HCOmath image secretion in class I mutations are of potential clinical interest.

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