Monitoring extracellular concentrations of lactate, glutamate, and glycerol by in vivo microdialysis in the brain during liver transplantation in acute liver failure

Abstract

Swelling of cerebral glial cells is a characteristic complication in patients with acute liver failure (ALF). This astrocyte edema may result in high intracranial pressure (ICP) and brain herniation before or during liver transplantation. Metabolic alterations responsible for the development of high ICP in patients with ALF are not fully understood. We describe changes in neurochemistry during liver transplantation using a cerebral microdialysis technique in a young man with severe ALF and cerebral edema. We found that the extracellular content of lactate ([lactate]_ec) gradually increased during the operation. Becauce cerebral oxygen saturation and [lactate]_ec to [pyruvate]_ec ratio were within normal limits, hypoxia was not likely to be responsible for the increased [lactate]_ec levels. Instead, we found that [lactate]_ec levels correlated in this patient with arterial lactate concentrations during and after grafting (r² = 0.96; P < .05), but did not correlate with arterial glucose concentrations (r² = 0.20; P = not significant). Also, [glutamate]_ec and [glycerol]_ec levels were severely elevated before liver transplantation, but tended to decrease in the hours after grafting. These findings indicate disturbances in glutamate neurotransmission, arachidonic acid metabolism, and lactate flux across the blood-brain barrier in patients with ALF.