Natural Hybridization in Saxifraga callosa Sm.
Article first published online: 14 FEB 2008
Volume 8, Issue 2, pages 243–252, March 2006
How to Cite
Grassi, F., Labra, M., Minuto, L., Casazza, G. and Sala, F. (2006), Natural Hybridization in Saxifraga callosa Sm. Plant Biology, 8: 243–252. doi: 10.1055/s-2005-873047
- Issue published online: 14 FEB 2008
- Article first published online: 14 FEB 2008
- Received: June 22, 2005; Accepted: November 10, 2005
- Saxifraga callosa;
- Saxifraga cochlearis;
Abstract: Saxifraga callosa Sm. is an evergreen perennial species distributed from Eastern Spain, through the Western Alps and the Apennines, to southern Italy. The existence of high morphological variation within different subspecies indicates that phenotypic characters are useful but not sufficient taxonomic tools. Indeed, available morphological data already suggested that S. callosa subentity lantoscana may be an outcross between S. callosa and S. cochlearis. In this work, by analyzing ITS (Internal Transcribed Sequences), AFLP (Amplified Fragment Length Polymorphisms), and cpDNA (chloroplast DNA) markers, a comprehensive study of the genomic relationships among S. callosa and related species has been carried out. The sequence of the ITS region of S. callosa subentity lantoscana gave no conclusive results on the taxonomy status of S. callosa subentity lantoscana. On the other hand, the use of the “NewHybrids” software to analyze an AFLP data-set (208 polymorphic amplified fragments) supported a significant posterior probability that S. callosa subentity lantoscana individuals are natural hybrids between S. callosa and S. cochlearis. The level of introgression of genes from alien genomes was confirmed by a simpler and quick methodology that analyze length variation in cpDNA sequences.