This work was supported by CNRS (UMR no 6612), the French Interdisciplinary Program “Small Animal Imaging” (2001–2002), ACI Plateformes d'Explorations Fonctionnelles Thématisées (2003), and Institut Universitaire de France. Isabelle Iltis was supported by a fellowship from Ministére de la Recherche.
Noninvasive Characterization of Myocardial Blood Flow in Diabetic, Hypertensive, and Diabetic–Hypertensive Rats Using Spin-Labeling MRI
Article first published online: 26 JAN 2010
Volume 12, Issue 8, pages 607–614, December 2005
How to Cite
ILTIS, I., KOBER, F., DALMASSO, C., COZZONE, P. J. and BERNARD, M. (2005), Noninvasive Characterization of Myocardial Blood Flow in Diabetic, Hypertensive, and Diabetic–Hypertensive Rats Using Spin-Labeling MRI. Microcirculation, 12: 607–614. doi: 10.1080/10739680500301607
- Issue published online: 26 JAN 2010
- Article first published online: 26 JAN 2010
- Received 11 January 2005; accepted 20 May 2005.
Objective: Microvascular alterations in the diabetic and hypertensive heart are likely to contribute to heart failure. In this work, myocardial blood flow and left ventricular function were measured in vivo in diabetic, hypertensive, and diabetic–hypertensive rats using MRI methods.
Methods: An 8-week-duration type 1 diabetes was induced by streptozotocin (STZ) in 8 Wistar-Kyoto (WKY) rats (STZ) and in 11 spontaneously hypertensive (SHR) rats (STZ-SHR). Fourteen WKY and 12 SHR served as control and hypertensive groups. Myocardial blood flow quantification was performed using an arterial spin-labeling MRI method. Left ventricular morphology and function were assessed during the same experiment using cine-MRI.
Results: Respective myocardial blood flow values for each group were 6.4 ± 1.1 (WKY), 6.0 ± 1.9 (STZ), 5.5 ± 1.3 (SHR), and 4.3 ± 0.9 mL· g−1· min−1 (STZ-SHR). Myocardial blood flow was significantly decreased in STZ-SHR rats compared with the other groups (p <.05, STZ-SHR vs. all groups). Cine-MRI showed morphological alterations in all pathological groups. No alteration of the ejection fraction was observed in the pathological groups.
Conclusion: Myocardial blood flow is altered in vivo before any sign of heart failure when rats have type 1 diabetes and hypertension simultaneously. When only one of the pathologies occurs, MBF does not vary significantly.