We gratefully acknowledge financial support from the NIH (R01 HL069957), and the Biological Resources Branch of the National Cancer Institute for providing VEGF for our studies. RC was supported by a predoctoral fellowship from the American Heart Association.
Host Immune Competence and Local Ischemia Affects the Functionality of Engineered Vasculature
Article first published online: 26 JAN 2010
Volume 14, Issue 2, pages 77–88, February 2007
How to Cite
CHEN, R. R., SNOW, J. K., PALMER, J. P., LIN, A. S., DUVALL, C. L., GULDBERG, R. E. and MOONEY, D. J. (2007), Host Immune Competence and Local Ischemia Affects the Functionality of Engineered Vasculature. Microcirculation, 14: 77–88. doi: 10.1080/10739680601131101
- Issue published online: 26 JAN 2010
- Article first published online: 26 JAN 2010
- Received 11 April 2006; accepted 10 July 2006.
- controlled drug delivery;
- growth factors;
- immune competence;
Objective: Localized and sustained delivery of vascular endothelial growth factor (VEGF) is a promising approach to overcome the limited efficacy of bolus delivery. The authors examined the effects of host immune competence and local ischemia on the functionality of new vessel networks formed with this approach.
Methods: Vessel structure and perfusion resulting from implantation of porous 85:15 poly(lactide-co-glycolide) scaffolds releasing VEGF165 were measured in both subcutaneous tissue and ischemic hindlimbs of immune competent C57BL/6 and immune deficient SCID mice.
Results: Sustained VEGF delivery resulted in a similar ∼100% increase in vessel density within scaffolds in both implant sites, and both animal models. However, the resulting perfusion within scaffolds implanted in subcutaneous tissue increased modestly versus control (18–35%), while perfusion increased 52–110% above control when VEGF-releasing scaffolds were placed in ischemic hindlimbs of C57BL/6 or SCID mice. VEGF delivery improved perfusion in the entire ischemic limb (55 ± 18% of the normal value by week 6; 138% increase over control) in SCID mice. Although C57BL/6 mice demonstrated spontaneous recovery from ischemia, VEGF delivery accelerated recovery as compared to control.
Conclusions: Localized and sustained VEGF delivery can create functional vasculature that amplifies recovery of tissue ischemia. However, increases in local and regional perfusion were highly dependent on the implantation site and the animal model.