S. Benesch and K. Rottner contributed equally to this work
Actin pedestal formation by enteropathogenic Escherichia coli and intracellular motility of Shigella flexneri are abolished in N-WASP-defective cells
Version of Record online: 1 SEP 2001
Copyright © 2001 European Molecular Biology Organization
Volume 2, Issue 9, pages 850–857, September 2001
How to Cite
Lommel, S., Benesch, S., Rottner, K., Franz, T., Wehland, J. and Kühn, R. (2001), Actin pedestal formation by enteropathogenic Escherichia coli and intracellular motility of Shigella flexneri are abolished in N-WASP-defective cells. EMBO reports, 2: 850–857. doi: 10.1093/embo-reports/kve197
- Issue online: 1 SEP 2001
- Version of Record online: 1 SEP 2001
- Manuscript Revised: 10 AUG 2001
- Manuscript Accepted: 10 AUG 2001
- Manuscript Received: 9 JUL 2001
Supplementary Video 1
Comparison of filopodia formation in a precursor (marked flox/flox) and a N-WASP-defective cells (del/del) upon microinjection of a mixture of L61Cdc42, N17Rac and C3-transferase (c3). The movie represents a time period of 32 min and microinjections were performed ∼2 min after start of the recording. The time period after injection is indicated. Note the dramatic induction of numerous filopodia in both cells. Bar represents 5 mm.
Supplementary Video 2
Reconstitution of Shigella-motility in N-WASP defective fibroblasts by ectopically expressed GFP-tagged full-length N-WASP (left) or H208D mutant (right). In both cases, motility is visualized by fluorescence of the respective fusion proteins localizing to the poles of bacteria. The video represents a time period of 23 min. Bar corresponds to 5 mm.