Cell therapy generates a favourable chemokine gradient for stem cell recruitment into the infarcted heart in rabbits
Version of Record online: 26 FEB 2009
Published on behalf of the European Society of Cardiology. All rights reserved. © 2009 the Authors
European Journal of Heart Failure
Volume 11, Issue 3, pages 238–245, March 2009
How to Cite
Lee, B.-C., Hsu, H.-C., Tseng, W.-Y. I., Chen, C.-Y., Lin, H.-J., Ho, Y.-L., Su, M.-J. and Chen, M.-F. (2009), Cell therapy generates a favourable chemokine gradient for stem cell recruitment into the infarcted heart in rabbits. European Journal of Heart Failure, 11: 238–245. doi: 10.1093/eurjhf/hfn035
- Issue online: 26 FEB 2009
- Version of Record online: 26 FEB 2009
- Manuscript Accepted: 24 NOV 2008
- Manuscript Revised: 13 OCT 2008
- Manuscript Received: 5 JUN 2008
- Stromal-derived factor 1;
- Bone marrow stromal cell;
- Myocardial infarction;
- Ventricular remodelling
Stem cell recruitment into the heart is determined by a concentration gradient of stromal-derived factor 1 (SDF-1) from bone marrow to peripheral blood and from blood to injured myocardium. However, this gradient is decreased in chronic myocardial infarction (MI). This study evaluated the effect of cell therapy using bone marrow stromal cells (BMSCs) on an SDF-1 gradient in post-infarction rabbits.
Methods and results
Myocardial infarction was induced in male New Zealand white rabbits (2.5–3 kg) by ligation of the left anterior descending coronary artery. Two months later, the rabbits were randomized to either saline or BMSC (2 × 106 autologous BMSCs injected into the left ventricular cavity) treatment. Four weeks after therapy, the SDF-1 gradients from bone marrow to blood and from blood to myocardium increased in the BMSC group compared with the saline group. This was accompanied by an increase in cells positive for CD34, CD117, and STRO-1 in the myocardium, resulting in more capillary density, better cardiac function, and a decrease in infarct size.
Generation of an SDF-1 gradient towards the heart is a novel effect of BMSC-based cell therapy. This effect facilitates stem cell recruitment into remodelled myocardium and supports improvement in cardiac function.